Opportunity Information: Apply for BOR UC 18 N019
The grant opportunity titled "Development of High-Throughput Markers for Rio Grande Silvery Minnow" (Funding Opportunity Number BOR UC 18 N019) is a discretionary funding announcement from the U.S. Department of the Interior, Bureau of Reclamation, focused on improving genetic monitoring tools for the endangered Rio Grande silvery minnow (RGSM). For nearly two decades, the RGSM genetics program has relied on a relatively small set of markers, specifically nine microsatellite loci plus the mitochondrial ND4 gene, to calculate genetic diversity metrics and inform management decisions. While that legacy approach has supported long-running monitoring since 1999, the opportunity is built around the need to modernize and expand the genetic toolkit so managers can meet current regulatory and scientific expectations.
A central driver behind the funding is compliance with the 2016 Final Biological and Conference Opinion covering Bureau of Reclamation, Bureau of Indian Affairs, and non-federal water management and maintenance activities in the Middle Rio Grande in New Mexico. That opinion explicitly requires ongoing genetic monitoring of the silvery minnow, and the Bureau of Reclamation is positioning high-throughput genetic markers as a way to satisfy that requirement more robustly. The project is also framed as a direct response to recommendations made by a Peer Review Panel that evaluated the RGSM genetic monitoring program in 2016, signaling that the agency wants an updated approach that reflects current best practices in conservation genetics rather than continued reliance on a small marker set.
The technical goal of the project is to develop a Single Nucleotide Polymorphism (SNP) panel using Restriction Site-Associated DNA Sequencing (RAD-seq). In practice, this means generating a much larger and more informative set of genetic markers distributed across the genome, which can improve the resolution and reliability of key population genetic analyses. Compared with microsatellites and a single mitochondrial gene region, a well-designed SNP panel can support more detailed and consistent estimates of genetic diversity through time and across locations, which is especially important for an endangered species managed through a mix of in-river (in situ) and captive or hatchery-associated (ex situ) actions.
The opportunity lays out three main objectives. First, the funded team is expected to produce a SNP panel with enough loci to support monitoring of spatiotemporal genetic diversity, estimation of effective population size, detection of population structure, and evaluation of relatedness within and among populations. This is meant to strengthen management decisions that depend on understanding how genetic variation is changing, whether genetic drift or bottlenecks are occurring, whether there are meaningful subgroups or differences among managed components, and how closely related individuals may be within breeding or supplementation programs. Second, the project must develop sex-specific genetic markers so that sex can be determined genetically, which can be useful when field identification is difficult, when working with early life stages, or when improving hatchery management and demographic modeling. Third, the award requires a strong reporting and coordination component, including submission of a draft study plan, delivery of oral presentations, and preparation of draft and final reports for agency review and approval, emphasizing that the work must be transparent, trackable, and aligned with agency needs throughout the project period.
The principal place of performance is identified as the University of New Mexico, and eligibility is limited to public and state-controlled institutions of higher education, indicating the award is structured to be carried out by an academic partner with the laboratory and analytical capacity to conduct RAD-seq development and downstream panel design. The funding listing indicates an award ceiling of $346,728, with one expected award, suggesting a single-institution or lead-institution effort rather than a large multi-award program. The opportunity falls under CFDA 15.517 and is categorized under Natural Resources, reflecting its conservation and species management purpose. The original posting timeline shows a creation date of September 5, 2018 and an original closing date of September 19, 2018, indicating it was a short application window aimed at moving quickly toward tool development that can be integrated into ongoing monitoring requirements.
Overall, the grant is essentially a targeted investment in upgrading the genetic monitoring infrastructure for an endangered fish in the Middle Rio Grande. By transitioning from a small traditional marker set to a validated high-throughput SNP panel and adding genetic sexing capability, the project is designed to deliver more actionable, higher-resolution information for conservation planning and compliance-driven monitoring, while also producing the formal plans and reports needed for agency oversight and adoption into the established RGSM monitoring program.Apply for BOR UC 18 N019
- The Department of the Interior, Bureau of Reclamation in the natural resources sector is offering a public funding opportunity titled "Development of High-Throughput Markers for Rio Grande Silvery Minnow" and is now available to receive applicants.
- Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.517.
- This funding opportunity was created on Sep 05, 2018.
- Applicants must submit their applications by Sep 19, 2018. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
- Each selected applicant is eligible to receive up to $346,728.00 in funding.
- The number of recipients for this funding is limited to 1 candidate(s).
- Eligible applicants include: Public and State controlled institutions of higher education.
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